Next, we prepared a digestion solution by mixing a 0.1% trypsin (Sigma-Aldrich, T1426-500MG, St. Louis, MO, USA) with the 0.035 M PBS solution. The sample was then placed in this digestion solution at ...
Afterwards, the proteins were alkylated by treating them via 50 mM IAA (Sigma-Aldrich, I6125) in 300 μL of UA buffer ... The proteins were subjected to enzymatic digestion using trypsin (Thermo Fisher ...
Cells were maintained in D10 medium [Dulbeccoʹs Modified Eagle Medium (DMEM) supplemented with 10% (v/v) foetal calf serum (Sigma-Aldrich, F9665), 2 mM glutamine ... VGM containing 0.5-1 µg/mL ...
Loss function of tumor suppressor FRMD8 confers resistance to tamoxifen therapy via a dual mechanism
Anti-Flag (#F3165) antibody was from Sigma-Aldrich. Antibodies against GAPDH (#AC002 ... For MS analysis, the protein in the gel was subjected to trypsin digestion. In the LC-MS/MS analysis, the ...
Where most cameras are built up around an internal chassis (typically magnesium alloy), Sigma instead opts for aluminum materials and a unibody build. Each BF camera is milled out of a solid block ...
On Monday, Sigma introduced its latest full-frame mirrorless digital camera—the BF—with a radically simple design. Featuring a 24.6 MP full-frame (35mm) sensor, it has just three physical buttons, ...
Find out how we test. Perhaps it’s an L-Mount Alliance in-joke, but the Sigma BF just out-Leicad Leica. Maybe the two companies placed a wager on whether Sigma would be first to outdo Leica’s cameras ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. As a common and severe cerebrovascular disease ...
LAS VEGAS, NEVADA - MARCH 01: (R-L) JJ Aldrich punches Andrea Lee in a flyweight fight during the UFC Fight Night event at UFC APEX on March 01, 2025 in Las Vegas, Nevada. (Photo by Jeff Bottari/Zuffa ...
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