Then, protein bands were washed twice in 25 mM AB. Proteolytic digestion was performed by addition of Trypsin (Promega, Madison, WI), 12.5 ng/µl of enzyme in 25 mM AB and incubated at 37 °C overnight.

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. In 2020–2021, a “mysterious illness” struck ...

Promega is the first to expand an STR multiplex to include eight dyes, making the kit more dependable for analyzing challenging or degraded DNA samples. Spectrum CE System, also developed by ...

RtcB enzymes are RNA ligases that play essential roles in tRNA splicing, unfolded protein response, and RNA repair. In metazoa, RtcB functions as part of a five-subunit tRNA ligase complex (tRNA-LC) ...

recombinant protein Trypsin Promega Cat#V5111 Commercial assay or kit BCA Protein Assay Kit Thermo Fisher Cat#23227 Commercial assay or kit Micro BCA Protein Assay Kit Thermo Fisher Cat#23235 ...