Treat the surviving cells with trypsin and plate them in 100-mm dishes ... Remove the medium from the cultured cells and wash them twice with calcium- and magnesium-free PBS, removing the final ...
Viruses were propagated by infecting a monolayer of MDCK-SIAT1 with ∼0.1 MOI (multiplicity of infection) virus for 1 h before replacing with VGM containing 0.5-1 µg/mL TPCK-treated trypsin ... were ...
Skimmed milk has a little more calcium than semi-skimmed, which in turn has a little more calcium than whole milk. The reason is simply that as the fat is removed, the watery proportion of the ...
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