It was anticipated that residual exterior imidazole carbamate linkers can be coupled to amine ... the peak predicted at 147.4 ppm is not seen in the literature reporting liquid NMR spectra for CDI in ...

Target proteins of aMOx and mutants were eluted by 200 mM imidazole. The buffer was replaced by using ... and equilibration periods, the NMR type of distance restraints were added between the vinyl ...

Solid-state NMR (ssNMR) spectroscopy is an ideal method for elucidating the structure, dynamics, and mechanism of action of viroporins in phospholipid membranes. This Account describes our ...

After 18 h, the cells were collected by centrifugation at 5000 × g for 20 mins and suspended in 35 mL of cold Binding buffer (50 mM HEPES-NaOH pH 7.5, 500 mM NaCl, 20 mM imidazole, 1 mM TCEP) and ...

His14-bdSUMO-MNase was eluted from the column with a linear gradient of imidazole (100 mM to 500 mM) in PBS. The fractions containing His14-bdSUMO-MNase were collected, mixed with SENP1 protease, and ...

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