Finally, trypsin was added at a 1:50 trypsin-to-protein mass ratio ... After being denuded of the zona pellucida using EmbryoMax Acidic Tyrode’s Solution (MR-004-D, Sigma-Aldrich), RNA-seq libraries ...

Trypsin and chymotrypsin have specificity pockets of essentially the same geometry, yet trypsin is specific for basic while chymotrypsin for bulky hydrophobic residues at the P1 site of the substrate.

Afterwards, the proteins were alkylated by treating them via 50 mM IAA (Sigma-Aldrich, I6125) in 300 μL of UA buffer ... The proteins were subjected to enzymatic digestion using trypsin (Thermo Fisher ...

Find out how we test. Perhaps it’s an L-Mount Alliance in-joke, but the Sigma BF just out-Leicad Leica. Maybe the two companies placed a wager on whether Sigma would be first to outdo Leica’s cameras ...

LAS VEGAS, NEVADA - MARCH 01: (R-L) JJ Aldrich punches Andrea Lee in a flyweight fight during the UFC Fight Night event at UFC APEX on March 01, 2025 in Las Vegas, Nevada. (Photo by Jeff Bottari/Zuffa ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. As a common and severe cerebrovascular disease ...

Next, we prepared a digestion solution by mixing a 0.1% trypsin (Sigma-Aldrich, T1426-500MG, St. Louis, MO, USA) with the 0.035 M PBS solution. The sample was then placed in this digestion solution at ...

On Monday, Sigma introduced its latest full-frame mirrorless digital camera—the BF—with a radically simple design. Featuring a 24.6 MP full-frame (35mm) sensor, it has just three physical buttons, ...

I've spent the past couple of days at Sigma headquarters and the Sigma factory in Japan, getting intimately acquainted with the new Sigma BF – perhaps the most beautiful camera I've ever seen, and one ...

Sigma has announced the 300-600mm F4 DG OS Sports lens, designed specifically for applications like sports, action and wildlife photography. Recent Videos Optically, the lens is built around 28 ...