Promega is the first to expand an STR multiplex to include eight dyes, making the kit more dependable for analyzing challenging or degraded DNA samples. Spectrum CE System, also developed by ...
Then, the membrane was incubated with anti-NLuc mouse monoclonal antibody (Promega; catalog no. N7000 ... Lonza) in FBS-free M199 supplement with 0.5 μg/ml of trypsin. Cultures were incubated for 7 ...
Residual solvent was removed in a vacuum centrifuge and an appropriate volume of a 10 ng/μL sequencing grade Trypsin (Promega) in 10% ACN, 40 mM ammoniumbicarbonate (ABC) were added. Digestion was ...
The mechanistic Target of Rapamycin complex 1 (mTORC1) senses intracellular amino acid levels through an intricate machinery, which includes the Rag GTPases, Ragulator and vacuolar ATPase (V-ATPase).