Then, protein bands were washed twice in 25 mM AB. Proteolytic digestion was performed by addition of Trypsin (Promega, Madison, WI), 12.5 ng/µl of enzyme in 25 mM AB and incubated at 37 °C overnight.

Residual solvent was removed in a vacuum centrifuge and an appropriate volume of a 10 ng/μL sequencing grade Trypsin (Promega) in 10% ACN, 40 mM ammoniumbicarbonate (ABC) were added. Digestion was ...

Cell viability was measured using CellTiter-Glo Luminiscent Cell Viability Assay (Promega, G7571) and Glomax Discover plate ... proteins were digested with LysC/trypsin overnight. Peptides were ...

It is designed for use with existing sequencing chemistries using fluorescently labeled dideoxynucleotide triphosphate and 4-, 5- and 6-dye STR kits from Promega and other commercially available kits.

The GloMax ® Discover is a ready-to-use multimode detection instrument developed with our reagent chemistries to provide a simple means of detecting advanced chemistries. The GloMax ® Discover ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Mycobacterial infections pose a significant ...

This valuable study offers insights into the role of Leiomodin-1 (LMOD1) in muscle stem cell biology, advancing our understanding of myogenic differentiation and indicating LMOD1 as a regulator of ...

Synthetic Biology of Photosynthetic Organisms, Matthias Schleiden Institute for Genetics, Bioinformatics and Molecular Botany, Friedrich Schiller University Jena, 07743 Jena, Germany ...

Research Analytical Biochemistry Laboratory, Department of Chemistry and Biology, Faculty of Science, Toronto Metropolitan University, 350 Victoria Street, Toronto, Ontario M5B 2K3, Canada ...