Purification by affinity chromatography was performed using a 5 mL Histrap HP or HiTrap Chelating HP in the ÄKTA purification system (Cytiva) using an increasing imidazole gradient between 10 and 500 ...
Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan 48824-1312, United States Department of Chemical Engineering, University of Michigan, Ann ...
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